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Applications of CRISPR-Cas9 for genetic engineering in parasitic protists
Ročeň, Milan ; Tachezy, Jan (advisor) ; Rada, Petr (referee)
The CRISPR / Cas system serves as a cellular defense mechanism that protects bacteria and archaea from foreign DNA, especially bacteriophages. Its product forms a ribonucleoprotein complex, whose components are sgRNA and Cas endonucleases. Using sgRNAs, which are complementary to foreign DNA, this complex recognizes the DNA and Cas endonucleases induce double-strand breaks. This method is applied both in primary research, where it is applicable for functional analysis of proteins and the study of gene expression, as well as in applied research, where it can apply, for example, in the production of genetically modified organisms or genetically attenuated vaccines. This work summarizes the current knowledge of the CRISPR / Cas system and its application for gene manipulation in parasitic protists.
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